Sanger sequencing

Sanger sequencing

Despite the introduction of NGS technologies over the past decade, Sanger sequencing remains the ‘gold standard’ to which other sequencing methods are compared.

It is widely used to confirm variants in genomic samples which have been detected using other NGS techniques, although its sensitivity limit of 20% Variant Allelic Frequency generally limits its use to hereditary genetics applications, in which variant alleles are present at either 50% or 100%.

We can work with you to determine the best approach to use, including design and synthesis of the necessary sequencing primer sets.

Contact us

NewGene Limited
Bioscience Building
International Centre for Life
Newcastle upon Tyne
NE1 4EP, UK

Tel: +44 (0)191 242 1923
Fax: +44 (0)191 241 8799
Email: info@newgene.org.uk

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Mon – Fri 08:30 to 17:00, closed Bank Holidays

An ISO 15189 accredited Medical Laboratory company

NewGene Limited | Company Number: 06735445

Technology Partners

Agilent Technologies

Agena Bioscience

Illumina

Sophia Genetics

Qiagen

Promega

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