Agena Bioscience MassARRAY and UltraSEEK genotyping

Agena Bioscience MassARRAY and UltraSEEK

Analysis of genetic profiles is an important element of many research projects. NewGene are an Agena Certified MassARRAY analytical services provider, having used the technology since the company was established in 2008.

Agena Massarray 4

MassARRAY is a rapid, sensitive and highly-multiplexed genotyping system which utilises a single base primer extension chemistry followed by MALDI-ToF analysis to probe for specific genotypes. This label free approach can analyse dozens of targets in one parallel reaction, making analysis very cost effective with a process time from sample to result of as little as 24 hours.

The standard iPlex analysis mode is well suited to genotyping of germlines and other solid sample types, detecting a proportion of variant alleles down to around 5% on a Wild Type background.

For rarer target sequences, an alternative analytical chemistry, UltraSEEK, is available. This approach utilises single base primer extension followed by capture and enrichment of target-specific reaction products using biotin-streptavidin chemistry. This approach substantially boosts the sensitivity of the assay, enabling detection of variant alleles down to 0.1% on a Wild Type background, making it particularly suitable for analysis of samples such as circulating tumour DNA isolated from blood plasma.

We are able to provide a range of services to suit project needs and customer preferences, ranging from a complete sample-to-report solution to instrument access, providing MALDI-ToF analysis of oligonucleotide reaction products produced in customer labs. Experimental data can be exported in a range of formats for further analysis, including .csv, .xlsx and .txt.

Contact us

NewGene Limited
Bioscience Building
International Centre for Life
Newcastle upon Tyne
NE1 4EP, UK

Tel: +44 (0)191 242 1923
Fax: +44 (0)191 241 8799
Email: info@newgene.org.uk

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Mon – Fri 08:30 to 17:00, closed Bank Holidays

An ISO 15189 accredited Medical Laboratory company

NewGene Limited | Company Number: 06735445

Technology Partners

Agilent Technologies

Agena Bioscience

Illumina

Sophia Genetics

Qiagen

Promega

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